The Study of Cytostatic Effect on MCF-7 Cells of the Alcohol Extract of Rhodiola Algida Var. Tangutica

摘要

Aims: To study the anti-tumor effect on MCF-7 cells and the related mechanism of the alcohol extract of Rhodiola algida Var. tangutica. Methods: The effective constituent of the extract was detected with the high efficiency liquid chromatography. The survival rate of MCF-7 cells was evaluated by MTT test. The morphological change of cells was observed with optical microscope. The apoptotic influence of the extract on cells was researched with Annexin VFITC/PI kit and flow cytometry. Results: The main constituent of the extract contained the salidroside and tyrosol. The extract caused decrease in the survival of MCF-7. The results of optical microscope showed the vacuole and apoptotic body in MCF-7 cells. The FCM analysis showed that lower concentrations of the extract caused much apoptotic cells and higher concentrations led to much necrotic cells. Conclusion: The extract of Rhodiola algida Var. tangutica inhibits division of MCF-7 cells and the cytostatic and antiproliferative effect was related to apoptosis induction mechanism.