Cloning and Expression Analysis of Wild Boar Osteopontin Gene

摘要

The wild boar OPN cDNA in size of 909bp was cloned by RT-PCR and analyzed by bioinformatics. The result showed that wild boar OPN contains 1) a potential calcium phosphate apatite binding region of eight consecutive Asp residues beginning at amino acid 86, 2) a cell-attachment Gly-Arg-Gly-Asp-Ser (GRGDS) sequence (residues 153-157), 3) a thrombin RS cleavage site (residues 163 and 164). The phylogenetic tree showed that the relationship between wild boar and domestic pig was the most close, but farthest with zebrafish, OPN gene accord with evolution relation. Wild boar OPN mRNA was abroad expressed in different tissue, higher in heart, stomach, lung, muscle, uterus, and lower in liver, kidney, small intestine and ovary. The protein weight was different in different tissue 70kDa, 70kDa and 45kDa, 70kDa, 45kDa and 24kDa.