摘要：
Objective To establish an ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with solid phase extraction (SPE) method for the determination of bisphenol A (BPA) and bisphenol S (BPS) in bottled drinking water.Methods With BPA-D4 and BPS-13C12 isotope as mixed internal standards,the BPA and BPS were enriched by Waters Oasis HLB column.The samples were separated by Waters BEH C18 chromatographic column with methanol-water as mobile phase,and then detected by UPLC-MS/MS under the multiple reactions monitoring (MRM) mode with the negative-ion mode and quantified by an internal standard method.Results Using the established method,BPA and BPS had good linearities in the ranges of 1-50 μg/L and 0.2-10 μg/L for BPA and BPS,respectively (r＞0.999).The average recoveries of BPA were 99.5％-100.7％ and the relative standard deviations were 0.8％-4.6％.The average recoveries of BPS were 95.9％-102.1％ and the relative standard deviations were 1.3％-2.5％.The least determination limits were 20 ng/L and 5 ng/L for BPA and BPS,respectively.Conclusion The established method is simple,sensitive and has good repeatability,which is suitable for the determination of BPA and BPS in bottled drinking water.%目的 建立固相萃取(solid phase extraction,SPE)结合超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)测定瓶装饮用水中双酚A(bisphenol A,BPA)、双酚S(bisphenol S,BPS)的方法.方法 利用BPA-D4和BPS-13C12混合同位素内标,经Waters Oasis系列的HLB固相萃取柱富集样品,以甲醇-水为流动相,经Waters BEH C18色谱柱分离,采用UPLC-MS/MS在负离子电离(electron spray ionization,ESI-)模式和多反应监控(multiple reaction monitoring,MRM)模式下检测.结果 用该方法测定时,BPA在1～50 μg/L范围内线性良好,BPS在0.2～10 μg/L范围内线性良好,相关系数r均为0.9999,BPA的平均回收率为99.5％～100.7％,相对标准偏差为0.8％～4.6％;BPS的平均回收率为95.9％～102.1％,相对标准偏差为1.3％～2.5％,BPA的最低检出浓度为20 ng/L,BPS的最低检出浓度为5 ng/L.结论 此方法操作简单、灵敏度高、重现性好,适用于瓶装饮用水中双酚A、双酚S的检测.
