Identificazione di Listeria Monocytogenes da alimenti tramite amplificazione genica e rilevazione colorimetrica in micropiastra (PCR-OSCPH)

摘要

This paper reports On an ELISA-based detection method for PCR-amplified Listeria monocytogenes iap gene fragment. During PCR, a label (digoxigenin-11 dUTP) is incorporated in the amplicon. After amplification, the product obtained is hybridized in streptavidin-coated microtiter plates prepared with biotinylated specific oligonucleotide as a DNA probe, and then an enzyme immunoassay reveals tile specifically bound complex and th is permits iclentification of L. monocytogenes. A total of 48 food santples were tested to validate the method involved. The PCR-OSCPH is easily applicable and much faster than traditional detection of L. monocytogenes in food, ntoreover, the hybridization in microtiter plates is more sensitive than routine agarose gel electrophoresis.