High-Throughput Measurement of the Enantiomeric Excess of Chiral Alcohols by Using Two Enzymes

摘要

The development of enantioselective catalytic processes has attracted increasing attention as a result of the importance of enantiomerically pure organic compounds in pharmaceutical, agricultural, and fine chemical syntheses. Attempts to discover new enantioselective catalysts have recently focused on the screening of libraries of chemical catalysts created by combinatorial synthesis, and enzymes and enzyme mutants generated by molecular biotechnology techniques such as the error-prone polymerase chain reaction and gene shuffling.