摘要：
目的:研究不同电针参数对大鼠弱精子症的治疗作用.方法:将105只雄性SD大鼠随机分为2 Hz-每日电针组9只、假电针组10只、模型组10只,2 Hz-隔日电针5d组、假电针组、模型组各8只,2 Hz-隔日电针9d组、假电针组、模型组各10只,100 Hz-隔日电针组7只、假电针组8只、模型组7只.采用奥硝唑灌胃法建立弱精子症大鼠模型.各电针组电针双侧“足三里”“肾俞”穴,各假电针组予常规针刺.2 Hz-每日电针组,每日治疗1次,共3次;2 Hz-隔日电针5d组,隔天治疗1次,共3次;2 Hz-隔日电针9d组,隔天治疗1次,共5次;100 Hz-隔日电针组,隔天治疗1次,共5次.通过计算机辅助精子分析系统观察电针对精子密度、活率、活力、A级精子数以及A+B级精子数的影响.结果:2 Hz-每日电针组:与模型组及假电针组相比,2 Hz-每日电针治疗对弱精子症大鼠精子运动的各项指标均没有明显改善作用(P＞0.05).2 Hz-隔日电针5d组:与模型组相比,2 Hz-隔日电针5d治疗可以增加大鼠精子的活力、A级精子数以及A+B级精子数(P＜0.05);而与假电针组相比,2 Hz-隔日电针5d治疗只增加了A+B级精子数(P＜0.05).2 Hz-隔日电针9d组:与模型组及假电针组相比,电针后弱精子症大鼠精子的活率、活力、A级精子数、A+B级精子数均显著升高(P＜0.001).100 Hz-隔日电针组:与模型组及假电针组相比,100 Hz-隔日电针5次治疗可提高大鼠精子的活率(P＜0.001,P＜0.05)、活力(P＜0.001,P＜0.01)、A级精子数及A+B级精子数(P＜0.01).各电针参数对弱精子症大鼠的精子密度无明显影响(P＞0.05).结论:2 Hz-隔日电针9d或100 Hz-隔日电针9d都可以提高弱精子症大鼠的精子活率和活力,提升精子运动能力,对弱精子症有一定的治疗作用.%Objective To investigate the effects of different electroacupuncture (EA) parameters for the treatment of asthenozoospermia in rats.Methods One hundred and five male Sprague-Dawley rats were randomly divided into 2 Hz-EA treatment daily in 3 d group (n=9),sham-EA group (n =10),model group (n =10);2 Hz-EA treatment every other day in 5 d group,sham-EA group,model group (8 rats in each group);2 Hz-EA treatment every other day in 9 d group,sham-EA group,model group (10 rats in each group);100 Hz-EA treatment every other day in 9 d group (n =7),sham-EA group (n =8),model group(n =7).Asthenozoospermia model was established by intragastric administration of ornidazole (ORN,400 mg · kg-1 · d-1) once daily till the end of treatment.EA treatments (2 Hz or 100 Hz) were applied to "Shenshu" (BL 23,bilateral),"Zusanli" (ST 36,bilateral) for 30 min,intensity of 1-2-3 mA (increasing 1 mA per 10 min),once a day or once every other day for 3 times or 5 times.Sham-EA groups were treated with similar procedure except that the output leads of the stimulator were disconnected.The sperm density,viability,motility,the number of grade A sperm,and grade A+ B sperm were examined by computer-assisted sperm analysis.Results (1) 2 Hz-EA treatment daily in 3 d:compared with the model group and the sham-EA group,2 Hz-EA treatment once daily had no significant effect on all of the sperm motility indexes in the asthenozoospermic rats (P＞0.05).(2) 2 Hz-EA treatment every other day in 5 d:compared with the model group,EA treatment could increase the sperm motility (P＜0.05),the number of grade A sperm (P＜0.05),and the number of grade A+ B sperm (P＜0.05) in the asthenozoospermic rats.However,compared with the sham-EA group,EA treatment could only improve the number of grade A + B sperm (P＜0.05).(3) 2 Hz-EA treatment every other day in 9 d:compared with both the model group and the sham-EA group,EA treatment could markedly improve the sperm viability (P＜0.001),the sperm motility (P＜0.001),the number of grade A sperm (P＜0.001),and the number of grade A+ B sperm (P＜0.001) in the asthenozoospermic rats.(4) 100 Hz-EA treatment every other day in 9 days:compared with both the model group and the sham-EA group,all of the sperm indexes in the asthenozoospermic rats including the sperm viability (P＜0.001 vs.the model group,P＜0.05 vs.the sham-EA group),the sperm motility (P＜0.001 vs.the model group,P＜0.01 vs.the sham-EA group),the number of grade A sperm (P＜0.01) and the number of grade A+B sperm (P＜0.01) also could be improved after EA treatment.Unexpectedly,none of the EA treatment had significant influence on the sperm density in the asthenozoospermic rats.Conciusion Both 2 Hz-EA and 100 Hz-EA treatment once every other day for 5 times in 9 d had a therapeutic effect on asthenozoospermia by improving the sperm viability and the sperm motility in the rats.
