Lysosome-mediated Cell Death and Autophagy-Dependent Multidrug Resistance in Breast Cancer

摘要

The original Statement of Work called for analysis of proteins and organelles involved in autophagic cell death (Task 1); for analysis of expression profiles and identification of pathways affected by tamoxifen treatment in parental cells and in resistant derivatives (Task 2); and for functional testing of identified differentially regulated genes as potential components of regulatory pathways for autophagy-related cell death (Task 3). It is important to recognize that Tasks 1 and 2 are parallel lines of investigation, so that Task 2 does not depend on completion of Task 1, and reversal of the timing of their execution does not affect execution of the overall project. By its nature Task 1 requires significant commitment of time (18 months projected) and is best performed by the same individual; on the contrary, Task 2 is associated mostly with analysis of data generated by expression profiling. Due to unforeseen delays, task 2 was begun before task 1.