Studies on Effect of EMS (Ethyl Methanesulphonate) on Papaya (Carica papaya L) Seeds under in-vitro Culture

摘要

The present experiment was carried out at Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (Uttar Pradesh) under two way Factorial experiments with three replications during 2011-12. Four varieties- Pusa Dwarf, Pant papaya 1,Pusa Giant and Washington were taken. The maximum seed germination (64.66%) was recorded in variety Pusa Giant and the minimum (63.22) in Washington. The maximum seed germination (92.50%) was recorded in control while the minimum (20.75%) was with 5000ppm of EMS treatment. The maximum and minimum plant heights were noted 28.69cm and 18.78cm in variety Pusa Giant and Pusa Dwarf, respectively at three month stage. The maximum plant height (26.57cm) was recorded under control which was followed by 2000ppm,lOOOppm and 3000ppm of EMS solution. The maximum as well as minimum stem girths (0.313cm and 0.246cm) were recorded in variety Pusa Giant and Pusa Dwarf, respectively. The maximum stem girth (0.336cm) was recorded under 2000ppm of EMS treatment. Maximumas well as minimum leaves (25.500 and 17.167) were noted in variety Pusa Giant and Pusa Dwarf, respectively. The maximum leaves (25.917) were recorded under 2000ppm of EMS solution.The maximum and minimum petiole lengths (22.667cm and 18.572cm) were noted under Pusa Giant and Pusa Dwarf varieties, respectively.The maximum as well as minimum petiole lengths were recorded under 2000ppm and 5000ppm of EMS treatment. As is evident that EMS @2000ppm was most effective for development of vigour rather than other concentrations where as 5000ppm of EMS drastically reduced the vigour of the plant. Thus, on observation of the data it was found that for development of ultra dwarf strains of papaya, higher concentrations of EMS will be effective for successful improvement of papaya cultivars.