High-Throughput Protein Glycomics:Combined Use of Chemoselective Glycoblotting and MALDI-TOF/TOF Mass Spectrometry

摘要

Glycosylation is one of the posttranslational modifications of proteins in eukaryotes.This step is thought to modulate a wide range of protein functions both on the cellular surfaces and within the cells.This highly dynamic process in which rapid changes in the carbohydrate structures occur in response to cellular signals or cellular stages result in the key informational markers of some serious human diseases.