Biochemical Composition and Immunological Comparison of Select Pecan [Carya illinoinensis (Wangenh.) K.Koch] Cultivars

摘要

On an edible portion basis,pecan moisture,protein,lipid,total soluble sugars,and ash contents ranged from 2.1% to 6.4%,6.0% to 11.3%,65.9% to 78.0%,3.3% to 5.3%,and 1.2% to 1.8%,respectively.With the exception of a high tannin (2.7%) Texas seedling,pecan tannin content was in a narrow range (0.6-1.85%).Unsaturated fatty acids (>90%) dominated pecan lipid composition with oleic (52.52-74.09%) and linoleic (17.69-37.52%) acids as the predominant unsaturated fatty acids.Location significantly influenced pecan biochemical composition.Pecan lipid content was negatively correlated with protein (r=-0.663) and total sugar (r=-0.625).Among the samples tested using SDS-PAGE a common pattern,with minor differences,in subunit polypeptide profiles was revealed.Rabbit polyclonal antibody-based immunoblotting experiments (Western blot) also illustrated the similarity in polypeptide profiles with respect to immunoreactivity.All tested cultivars registered similar immunoreactivity when their protein extracts (each at 1 mg/mL) were assessed using inhibition ELISAs (mean ± standard deviation=0.89 ± 0.20;n=27) with the USDA "Desirable" cultivar as the reference standard (immunoreactivity designated as 1.0).