Sensing Phosphatase Activity by Using Gold Nanoparticles

摘要

Phosphorylation and dephosphorylation play pivotal roles in cellular regulation and signaling processes.Numerous inhibitors have been proposed or applied to regulate the process of phosphorylation/dephosphorylation.A sensitive assay to report change of the phosphorylation state will be extremely valuable for biomedical applications.As the chemical difference in the process is only a phosphate group,developing a simple and sensitive assay is challenging.Herein we present a novel design of a gold-nanoparticle-based colorimetric assay to detect phosphatase activity.