Differential Analysis of 2D NMR Spectra:New Natural Products from a Pilot-Scale Fungal Extract Library

摘要

The efficient analysis of small-molecule mixtures underlies many endeavors in chemical biology.The sensitivity of mass spectrometry (MS) has resulted in its widespread adoption for such analyses,and today rapid automated LC-MS analyses are widely used.Several recent studies have demonstrated the feasibility of NMR spectroscopic analyses of complex small-molecule mixtures,including the use of diffusion-ordered spectroscopy (DOSY) or principal component analysis (PCA) in metabolomics,as well as the characterization of crude unfractionated natural product extracts using routine two-dimensional NMR spectra.