Effect of Aeration-Agitation on Coenzyme Q_(10) Production Using Rhodobacter sphaeroides

摘要

With the aim of increasing the CoQ_(10) production in mass culture, the effect of aeration-agitation on the CoQ_(10) production using Rhodobactor sphaeroides was investigated in a 1-L bioreactor. The maximum CoQ_(10) production was 1.69 mg/g of dry cell weight under conditions of 50 Lux, 30 deg C, 300 rpm, and 5-vvm aeration. The CoQ_(10) production was improved to produce 2.91 mg/g of dry cell weight under reduced conditions of agitation speed (200 rpm) and aeration rate (0.2 vvm). When R. sphaeroides was cultivated under more reduced DO levels during the exponential phase of the cell, the CoQ_(10) production yield of 3.88-mg/g dry cell weight was the maximum obtained. Therefore, poorer conditions of aeration-agitation resulted in higher productionof CoQ_(10), and thus DO content was a crucial factor in the production of CoQ_(10). Accordingly, it was necessary to control the DO concentration in order to enhance the CoQ_(10) biosynthesis within a large-scale production.