Synthesis, characterization, photocleavage, antimicrobial activity and DNA binding of [Co(bpy)_2MHPIP]~(3+), [Co(dmb)_2MHPIP]~(3+), and [Co(phen)_2MHPIP]~(3+) complexes

摘要

4-Methyl-2-(2-hydroxyphenyl)imidazo[4,5-f][l,10]phenanthroline) (MHPIP) and its complexes [Co(bpy)_2MHPIP]~(3+) (1) (bpy = 2,2'-bipyridine), [Co(dmb)_2MHPIP]~(3+) (2) (dmb = 4,4'-dimethyl-2,2'-bipyridine), and [Co(phen)_2MHPIP]~(3+) (3) (phen= 1,10-phenanthroline) have been synthesized and characterized by UV/VIS, IR, EA, ~1H, ~(13)C-NMR, and mass spectra. The binding of the three complexes with calf-thymus-DNA (CT-DNA) has been investigated by absorption and emission spectroscopy, DNA-melting techniques, viscosity measurements, and DNA cleavage assay. The spectroscopic data and viscosity results indicate that these complexes bind to CT-DNA via an intercalative mode. The complexes also promote photocleavage of plasmid pBR322 DNA and were screened for antimicrobial activity.