Concatenation of near- and mid-infrared spectra to improve calibrations for determining forage composition.

摘要

This study determined the usefulness of combining near- and mid-infrared diffuse reflectance spectra to improve partial least squares calibrations. A set of 174 chlorite-treated feedstuffs were assayed chemically and spectroscopically for fibre, crude protein, lignin, digestibility and lignin composition. All samples were scanned by diffuse reflectance in the near- and mid-infrared using a Fourier transform spectrometer equipped with a sample transport (to increase the amount of sample scanned) under various instrument configurations. Partial least squares analysis showed little or no improvement in calibration results by combining near- and mid-infrared spectra, although results using both regions together were at times better than those obtainedusing 1 of the 2 regions. It was concluded that combining data from the near- and mid-infrared spectral regions offers little or no advantage over the use of a single spectral range.