Characterization of the mobilization region of the Zymomonas mobilis ATCC10988 plasmid pZMO3.

摘要

The 2.7-kb Zymomonas mobilis ATCC10988 plasmid pZMO3 contains a coding region (ORF1) indispensable for mobilization. A cis-acting 409-bp sequence between ORF2 (C-terminal) and ORF1 (N-terminal) conferred mobilization activity to pUC19, when the product of ORF1 was provided in trans. In this area, two segments showed homology with previously characterized oriT regions. Copyright 1999 Academic Press.