Detection of Goat pox Antibodies: Comparative Efficacy of Indirect ELISA and Counterimmunoelectrophoresis

摘要

Bhanuprakash, V., Hosamani, M., Juneja, S., Kumar, N. and Singh, R.K. 2006. Detection of goat pox antibodies: comparative efficacy of indirect ELISA and counterimmunoelectrophoresis. J. Appl. Anim. Res., 30:177?￠????180. An indirect ELISA was optimized and evaluated using purified goat pox virus (GPV, cell culture attenuated) antigen and different groups of serum samples from goats with known and unknown immune status for the detection of GPV antibodies. The cut-off value higher by 0.2 at A 492 of the control negative sera gave 90% sensitivity and 94.26% specificity for the assay. Of the total 1708 goat serum samples screened, 1054 (61.70%) were positive in indirect ELISA, where as only 219 (12.82%) samples were positive in counterimmunoelectrophoresis (CIE) test, a preliminary diagnostic test for goat pox. The indirect ELISA was observed to be superior to CIE test in the detection of GPV antibodies in the goat sera and its use has been recommended.