Comparative evaluation of culture, ELISA and PCR in screening of milk samples for the detection of Mycobacterium avium subsp. paratuberculosis infection in lactating goats

摘要

Prevalence of MAP infection in goat's milk of organized and farmer's herds ws studied using culture and PCR and plate-ELISA. Milk samples (53) were from (3 goat units) of organized herds (CIRG), whereas 255 milk samples from CIRG and 67 from nearby villages were tested by milk ELISA. Fat and sediment layers were cultured and DNA was isolated and whey tested for ELISA. Antigen was prepared from Mycobacterium avium subsp. paratuberculosis (bison type) strain S 5 of goat origin. DNA isolated from decontaminated milk samples, was amplified using IS 900 primers. PCR and products were of 229 bp as in case of standard MAP S 5 (Bison type) strain.