Real-Time Binding Kinetics Monitored with Surface Plasmon Resonance Imaging in a Diffusion-Free Environment

摘要

Surface Plasmon Resonance Imaging (SPRI) is an optical technique emerged as a powerful tool for the simultaneousmonitoring of interactions of biomolecules arrayed onto gold substrates. To take fully advantage of the SPRI approacha precise control of the fluidic of the analyte solution is imposed. The diffusion between the flowing buffer and theanalyte solution which is established within the fluidic system creates a liquid volume where a gradient in the analyte concentrationis present. Such gradient is shown to affect the kinetics parameters obtained from the SPRI response. We presentresults obtained from a new delivery approach based on the use of air bubbles. The advantages offered by the newapproach are demonstrated by using two different interacting biomolecular systems: the streptavidin-biotin system and theRicinus Communis Agglutinin lectin-asialofetuin.