乌贼皮胶原蛋白的提取及结构表征

摘要

为了充分利用乌贼加工废弃物,分析了乌贼皮的基本组成成分,优化了从乌贼皮中提取胶原蛋白的工艺条件,并利用SDS-PAGE垂直电泳、紫外扫描和傅里叶变换红外光谱对所提取的胶原蛋白进行了结构表征.结果表明,乌贼皮中含有大量胶原蛋白,可作为胶原蛋白来源的补充.采用酸酶复合提取胶原蛋白的最佳条件为:酒石酸浓度为0.1mol/L,胃蛋白酶添加量为1400U/g,料液比为1:20(m:V,原料),4℃提取18h,提取率为12.08％.SDS-PAGE垂直电泳、紫外扫描和傅里叶变换红外光谱的结果表明,采用酸酶复合法从乌贼皮中提取的胶原蛋白为I型胶原蛋白,保持了完整的三螺旋结构.%In order to take full advantage of cuttlefish (Sepia esculenta) processing waste, the basic components of cuttlefish skin were analyzed and the extraction conditions for collagen from cuttlefish skin were optimized. Along with this,purified collagen was analyzed through SDS-PAGE vertical electrophoresis, UV scanning and FT-IR. The results showed that cuttlefish skin contained large amounts of collagen and it could be used as the source of complement for collagen. The optimum conditions for collagen with acid and pepsin were determined as follows ： soaking cuttlefish skin in 0.1 mol/ L tartaric acid solution with a ratio of material to liquid of 1=20 (m: V,raw materials) at 4 ℃ for 18 h,the 1400 U/g pepsin was added in the beginning for the restriction hydrolysis,and the extraction yield of collagen was 12.08%. SDS-PAGE vertical electrophoresis, UV scanning and FT-IR results indicated that the extracted collagen was type I collagen and the unique triple helical structure of type I collagen remained well.