“黑美人”土豆色素体外抗氧化性研究

摘要

采用普鲁士蓝法、DPPH清除体系、H2O2/Fe2+/水杨酸检测体系、亚硝基清除体系、卵黄脂蛋白不饱和脂肪酸(PUFA)过氧化体系、邻苯三酚自氧化法研究了黑美人土豆色素的体外抗氧化活性,并同VC进行了比较.结果表明,黑美人土豆色素对几种自由基均有不同的清除作用,其中黑美人土豆色素的还原力、抗脂质过氧化能力、清除羟基自由基、亚硝基、超氧阴离子自由基(O2-·)和DPPH自由基的能力均高于VC.该结果显示,黑美人土豆色素是一种较好的天然自由基清除剂,可以作为绿色食品用于人们的日常饮食,有较大的开发利用潜能.%The antioxidant activity of pigment from black beauty potato was studied in vitro by prussian blue method, DP-PH scavenging system,H2O2/Fe2+ salicylic acid detection system,nitroso removal system,the yolk lipoprotein polyunsat-uiated fatty acids (PUFA) peroxidation system, pyrogallol autoxidation system,and compared with that of vitamin C. The results showed that the pigment of black beauty potatoes had different ability of scavenging on different free radicals, of which the reducing capacity, anti-lipid peroxidation, ability of scavenging hydroxyl radical, nitroso, superoxide anion (O2--' ) ,and DPPH radical were higher than VC. The results indicate that the pigment of black beauty potato is a good natural free radical scavenger. Therefore the black beauty potato can be used as Green food in daily diet,and it has great potential of development and utilization.