大鼠原代肝星状细胞活化过程中CCN家族表达研究

摘要

Liver fibrosis is the pathological repair response of liver to injury and characteristic of activation and expanding of hepatic stellate cells (HSC) and excessive accumulation of extracelluar matrix proteins (ECM) in liver.CCN factors are the signaling matricellular protein,involving in tissue repair and fibrogenesis.In this study,the highly pure primary rat HSC was isolated and cultured for their transition into the myofibroblastic phenotype.These cultured HSCs were daily collected and characterized for the MF phenotypic features,and then mRNA expression of all CCNs genes within these transiting HSCs were analyzed with the real time QRT-PCR for a full assessment of the roles of CCNs during HSC trans-differentiation.%肝纤维化是肝脏损伤后的病理性修复反应,其特征是肝脏中肝星状细胞(HSC)激活、扩增和细胞外基质蛋白过度沉积.CCN蛋白因子是具信号传递功能的细胞基质蛋白,参与组织修复反应并涉及肝纤维化发生发展.本文制备了高纯度的大鼠原代HSC并通过体外培养进行激活,同步定量分析了6个CCN因子在激活过程中的表达,以期对CCN因子在HSC激活过程中的作用进行更全面的评价.