三花枪刀药中萜类和甾体成分研究

摘要

In this study,15 compounds were isolated and purified from the 95％ EtOH extract from Hypoestes triflora by column chromatography on silica gel,Sephadex LH-20 and HPLC.Their structures were elucidated as 6-hydroxy-9,13-cyclomegastigma-4,9 (13)-dien-3-one (1),3β-hydroxy-β-ionone (2),3 α-hydroxy-megastigrna-4,7E-dien-9-one (3),5α,6α-epoxy-3α-hydroxy-megastigma-7E-en-9-one (4),loliolide (5),pisiferadinol (6),24-methylenecycloartanol (7),α-amyrin (8),3β-hydroxyurs-11-en-28,13β-olide (9),lupeol (10),achilleol A (11),6β-methoxyergosta-7,22E-dien-3β,5 α-diol (12),6β-ethoxyergosta-7,22E-dien-3β,5 α-diol (13),stigmast-4-en-3,6-dione (14),stigmast-4,22E-dien-3,6-dione (15),by modem spectroscopic methods.Compound 1 was isolated from nature resources for the first time,and compounds 2-15 were obtained from H.triflora for the first time.Compounds 6,12,15 showed significant cytotoxic activities against MDA-MB-468,AGS,HCT116,Hela,and MDA-MB-231 cells.%采用正相硅胶和Sephadex LH-20等柱层析及半制备HPLC色谱法,从三花枪刀药的95％乙醇提取物中分离到15个化合物,运用现代波谱技术分别鉴定为6-羟基-9,13环大柱烷4,9(13)-二烯-3-酮(1)、3β-羟基-β-紫罗酮(2)、3α-羟基-大柱烷-4,7E二烯-9-酮(3)、3α-羟基-5α,6α-环氧-7E-大柱烷-7-烯-9-酮(4)、黑麦草内酯(5)、pisiferadinol(6)、24-methylenecycloartanol(7)、α-香树脂醇(8)、3β-羟基-乌苏烷-11-烯-28,13β-内酯(9)、羽扇豆醇(10)、achilleol A(11)、6β-甲氧基麦角甾烷-7,22E-二烯-3β,5α-二醇(12)、6β-乙氧基麦角甾烷-7,22E-二烯-3β,5α-二醇(13)、豆甾烷-4-烯-3,6-二酮(14)、豆甾烷-4,22E-二烯-3,6-二酮(15).化合物1为新天然产物,化合物2～15为首次从该植物中分离得到.化合物6、12、15对人乳腺癌MDA-MB-468、人胃癌AGS、人结肠癌HCT116、人宫颈癌Hela和人乳腺癌MDA-MB-231肿瘤细胞株具有显著的细胞毒活性.