分别以石油醚、80％乙醇、水为溶剂,依次对银杏外种皮中的银杏酸、黄酮以及多糖进行了联合提取研究.结果表明:常温条件下银杏外种皮经高速剪切破壁,石油醚提取银杏酸40 min,2次累计提取率可达98.5％,银杏酸含量可达54.6％,经硅胶柱纯化后纯度可达到97.5％;残渣用80％乙醇提取黄酮60 min,固液比1:15,1次提取率可达94.6％,其中总黄酮含量为7.5％,经水沉、大孔树脂纯化,黄酮苷含量为24.4％;最后以水为溶剂,90℃下回流提取剩余残渣120 min,固液比为1:10,多糖2次累计提取率可达72.7％,含量为24.3％,除去蛋白纯化后纯度可达64％.%Petroleum ether,80％ of ethanol and water were respectively used as solvent forintegrated extraction of ginkgo acid,flavone and polysaccharide from Ginkgo biloba sarcotesta successively.The result showed that:under the ambient temperature conditions,the cytoderm of Ginkgo biloba sarcotesta had been broken by high-speed sheafing,petroleum ether was used to extract ginkgo acid for 40 min,two-time accumulativc extraction rate reached 98.5％,the content reached 54.6％.Its purity after purification with silica gel column chromatography reached 97.5％;then 80％ ethanol was used to extract flavonefor 60 min from residue,solid-to-liquid ratio was 1:15,the one-timeextraction rate reached 94.6％,the content of total flavone was 7.5％,the content of flavonoid glycoside was 24.4％ after water precipitation and purification with macroporous resin column chromatography;at last,water was used as solvent to reflux extract the rest residue for 120 min under 90 ℃,solid-to-liquid ratio was 1:10,two-time accumulative extraction rate of polysaccharide reached 72.7％,the content was 24.3％,the purity reached 64％ after purification by removing protein.
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