上皮性卵巢癌中微管驱动蛋白KIF2 A的表达及其意义

摘要

目的 检测微管驱动蛋白KIF2A及其mRNA在卵巢癌中的表达情况,检测转染KIF2A-siRNA卵巢癌细胞(HO-8910)在迁移和侵袭能力方面的变化,探讨KIF2A在卵巢癌细胞恶性行为中所起的作用.方法 应用免疫组织化学方法检测了KIF2A在30例卵巢癌和20例卵巢正常组织中的表达,同时应用逆转录-聚合酶链反应(RT-PCR)法检测了KIF2A mRNA的表达情况.体外培养HO-8910,KIF2A-siRNA干扰KIF2A表达后,应用RT-PCR和Western 印迹检测细胞系中KIF2A表达变化;分别用细胞划痕、transwell 的实验方法检测干扰KIF2A作用后对细胞迁移和侵袭行为的影响.结果 KIF2A在卵巢癌中的表达显著高于正常卵巢组织(P<0.05);KIF2A-siRNA干扰后,HO-8910中KIF2A mRNA及蛋白水平显著下调(P<0.05),细胞迁移和侵袭能力被显著抑制(P<0.05).结论 KIF2A在卵巢癌组织中表达升高,干扰KIF2A基因的表达可抑制卵巢癌细胞的迁移和侵袭能力,提示KIF2A基因可能成为干预卵巢癌发展的新靶点.%Objective To investigate the protein and mRNA expression of KIF 2A in ovarian cancer,and to investigate the migration and invasion ability changes in ovarian cancer cell line HO -8910 transfected by KIF2A-siRNA.Methods Immunohistochemical method was used to detect the expression of KIF2A in 30 cases of ovarian cancer and 20 cases of ovarian normal tissues.The expression of KIF2A mRNA was detected by RT-PCR.The mRNA and protein expression of KIF2A in cell line HO-8910 was detected by RT-PCR and Western blot after transfected by KIF 2A-siRNA in vitro.After the transfection, the cell migration and invasion ability were observed by scratch test and transwell experiments.Result The expression of KIF2A mRNA and protein in HO-8910 was significantly lower than that in normal ovarian tissue (P<0.05).The capacities of migration and invasion of HO-8910 was suppressed notably after the knockdown of KIF2A(P<0.05).Conclusion KIF2A gene expression was increased in ovarian cancer, and knockdown of KIF2A gene can inhibit the migration and invasion of ovarian cancer cells.It suggested that KIF2A gene may be a new target for the development of ovarian cancer.