β-连环蛋白在KB细胞及其肿瘤球细胞中的表达

摘要

Objectivf :To study the expression of signaling pathway β - catenin in human KB cell line and tumor sphere cell. Methods : KB and KB tumor sphere cells were cultured . RNA was extract respectively from KB cell and KB tumor sphere cell . The mRNA of β - catenin was detected by RT - PCR with primer designed by ourselves. The protein of β - catenin was detected by western. Resulsts : β - catenin of two kind cell were positive, but the mRNA amount of KB cell was higher than tumor sphere cell. Western results showes two kind cell were positive, but the protein amount of tumor sphere cell was higher than KB cell. Conclusion : β - catenin protein is accumulated and transcrihed in KB cell and KB tumor sphere cell.%目的:研究信号通路β-连环蛋白在人口腔上皮样癌KB细胞及其肿瘤球细胞中的表达.方法:培养KB细胞及其肿瘤球,分别提取KB细胞和肿瘤球细胞的总RNA,设计引物,使用RT-PCR方法检测β-连环蛋白在KB细胞和肿瘤球细胞中的转录,使用Western blot方法检测β-连环蛋白在两种细胞中的翻译.结果:RT-PCR方法检测显示两种细胞的β-连环蛋白的结果均为阳性,KB细胞中β-连环蛋白 mRNA含量多于肿瘤球细胞.Western bolt方法检测显示结果均为阳性,KB细胞中β-连环蛋白含量少于肿瘤球细胞.结论:在KB细胞及其肿瘤球细胞中β-连环蛋白转录并积累.