人KB细胞和CCL227细胞与肿瘤球细胞超微结构的比较

摘要

目的:比较人口腔癌KB细胞和结肠癌CCL227细胞与肿瘤球细胞的超微结构,为肿瘤干细胞研究提供更多的资料.方法:使用有血清培养液培养两种肿瘤细胞,无血清培养液分别诱导两种肿瘤细胞生成肿瘤球.常规方法制备KB细胞、CCL227细胞、KB肿瘤球细胞、CCL227肿瘤球细胞透射电子显微镜切片,透射电子显微镜观察、拍照.结果:在四组样本中,细胞基本表现两种形态.一种细胞表面有突起,核糖体、线粒体数量相对较多.另一种细胞表面无突起,细胞核周围出现微丝,核糖体、线粒体数量减少,核内染色质出现靠边,后一种细胞在肿瘤球细胞中较多见.结论:伴随生理功能的改变,肿瘤干细胞的结构也发生了一些改变,这些改变可能会使蛋白合成下降.%Objective: To compare the ultrastructure of tumor cell and tumor sphere cell in human KB cell line and CCL227 cell line. Methods: SSM was used to culture KB cells and CCL227 cells. The SFM was used to induce KB tumor sphere and CCL227 tumor sphere. The sections of transmission electron microscope of KB cell, CCL227 cell, KB tumor sphere cell, CCL227 tumor sphere cell were made by routine method, and were observed and photographed. Results: The cells displayed two kinds of morphous roughly. One kind cell had surface claw, more ribosome andmore mitochondrion. Another cell had not surface claw, less ribosome and mitochondrion, and more microfilaments appear around cell nucleus. Chromatin in cell nucleus keep to the side. Moreover, the amount of the latter was more in tumor sphere cell than tumor cell. Conclusion: The structure of tumor stem cell has some changes with the change of the physiological functions. These change maybe result in a drop of protein synthesis.