苦参碱对宫颈癌 SiHa 细胞增殖的抑制作用及 JAK - STAT 信号通路的影响

摘要

目的：观察苦参碱对宫颈癌 SiHa 细胞 JAK - STAT 信号通路的影响。方法：苦参碱作用于宫颈癌 Si-Ha 细胞。用 MTT 法检测不同浓度苦参碱对 SiHa 细胞增殖的抑制作用。Western blot 法检测不同浓度苦参碱对宫颈癌细胞 JAK1、JAK2和 STAT3蛋白表达的影响。结果：苦参碱能够抑制宫颈癌 SiHa 细胞的增殖，呈时间-剂量依赖性。苦参碱能降低 JAK1、JAK2和 STAT3蛋白表达量（P ﹤0.01），有剂量依赖性。结论：苦参碱对宫颈癌 SiHa 细胞有抑制作用。其作用机制可能为下调宫颈癌细胞 JAK1、JAK2、STAT3蛋白表达水平，阻断细胞信号转导通路，从而抑制宫颈癌细胞增殖。%To investigate the effects of matrine on the JAK - STAT signaling transduction pathways in cervical cancer - derived SiHa cells. Methods:Treated with different concentrations of matrine solution for 24h,48h and 72h,the cytotoxic effect of matrine on SiHa cells was assessed with MTT assay. The protein expression of JAK1, JAK2,STAT3 in SiHa cells were detected by Western blot. Results:Different concentrations of matrine were cytotoxic to SiHa cells in a time - dependent and dose - dependent manner. The protein expression of JAK1,JAK2,STAT3 in SiHa cells with matrine was significantly lower than those in control group(P ﹤ 0. 01). Conclusion:Matrine has a de-pressive role in the proliferation of cervical cancer - derived SiHa cells. Its mechanism is related to down - regulating the protein expression of JAK1,JAK2,STAT3 and inhibiting the signaling transduction pathway of JAK - STAT.