卡铂耐药蛋白S100 A4在上皮性卵巢癌中的表达及与化疗耐药的关系

摘要

Objective:To explore the relationship between chemoresistance and S100A4 expression in epithelial o-varian carcinoma. Methods:The expression of S100A4 was detected by SP immunohistochemistry method in 81 epi-thelial ovarian carcinoma which were divided into resistant group(40 cases)and sensitive group(41 cases). Western Blot was used to identify S100A4 protein level respectively carboplatin sensitive SKOV3 cell line and carboplatin re-sistant SKOV3/CB cell line. 50% inhibitory concentration( IC50 )to carboplatin in SKOV3 and SKOV3/CB was de-tected with MTT method. Results:The expression rate of S100A4 in epithelial ovarian carcinoma carboplatin resistant was higher than that in epithelial ovarian carcinoma carboplatin sensitive(P﹤0. 05). The expression of S100A4 had correlation with chemotherapy resistance in epithelial ovarian carcinoma. Both cytoplasmic and nuclear expression or nuclear expression of S100A4 in epithelial ovarian carcinoma carboplatin resistant was significantly higher than that in than those in epithelial ovar-ian carcinoma carboplatin sensitive(P﹤0. 05). The IC50 was 28. 83μmol/L in SKOV3 cells. The IC50 was 69. 11μmol/L in SKOV3/CB cells. Multidrug resistance index of carboplatin resistant SKOV3/CB was 2. 4. Conclusion:The expression of S100A4 is closely associated with chemotherapy-resistance of epithelial o-varian carcinoma and provide certain evidence for indivi-dualized treatment in ovarian cancer patients.%目的：检测S100A4蛋白在上皮性卵巢癌中的表达及其与化疗耐药的关系。方法：选择81例卵巢上皮性癌组织，分为耐药组40例，敏感组41例，应用免疫组织化学法检测S100A4在卵巢上皮性癌组织中的表达情况；MTT法测定卡铂对细胞的半数抑制浓度（ IC50），采用Western Blot检测卡铂化疗敏感的卵巢癌细胞株SKOV3及卡铂化疗耐药的卵巢癌细胞株SKOV3/CB中S100A4的表达情况。结果：S100A4分别在卵巢癌组织和卵巢癌细胞耐药组中的表达高于敏感组，与卵巢癌的化疗耐药有关（ P﹤0.05）。S100A4在胞浆/胞核或胞核表达耐药组中高于敏感组，有统计学意义。卡铂对 SKOV3组和 SKOV3/CB 组的 IC50分别为28.83μmol/L和69.11μmol/L，因此SKOV3/CB对卡铂的耐药指数（ RI）为2.4。结论：S100A4的表达与上皮性卵巢癌的化疗耐药密切相关，为卵巢癌患者个体化治疗提供依据。