miR-873抑制肝细胞癌的侵袭与迁移

摘要

目的:检测miR-873在肝细胞癌(hepatocellular carcinoma,HCC)中的表达情况,探究其与HCC临床病理特征及HCC病人预后的相关性,进一步探究其对HCC细胞侵袭与迁移的影响.方法:应用Real-time PCR法检测90例HCC组织和对应癌旁组织中miR-873的表达水平;应用统计学方法分析miR-873的表达水平与HCC临床病理特征的相关性及对HCC病人预后的影响;应用Real-time PCR法检测HCC细胞系中miR-873的表达水平,并应用Transwell小室实验探究miR-873对HCC细胞侵袭与迁移能力的影响.结果:miR-873在90例HCC组织中的表达水平较癌旁组织显著下调(P<0.05);miR-873的表达水平与肿瘤数目、静脉侵犯、Edmondson病理分级及TNM分期显著相关(P<0.05);Kaplan-Meier曲线分析发现,miR-873低表达的患者生存率较差(P<0.05),且miR-873可作为肝癌病人术后生存的独立预测指标;miR-873在HCC细胞系中(Hep3B、MHCC-97H、HepG2、SMMC-7721)的表达较正常肝细胞LO2显著下调(P<0.05);Transwell小室实验显示miR-873能显著抑制HCC细胞的侵袭与迁移能力.结论:miR-873在HCC中表达下调,与HCC的临床病理及HCC病人的预后相关,并且能够抑制HCC细胞的侵袭与迁移能力.%Objective:To detect the expression level of miR-873 in hepatocellular carcinoma,and explore the clinical significance and functions of miR-873 in invasion and metastasis of hepatocellular carcinoma.Methods:Re-al-time PCR was performed to investigate the miR-873 expression in 90 paired HCC tissues and adjacent normal tissues.The clinical significance of miR-873 and its effects on HCC patients prognosis were analysed by statistics a-nalysis.Real-time PCR was performed to detect the miR-873 expression in HCC cell lines.Transwell assay was ap-plied to explore the functions of miR-873 in metastasis and invasion of HCC.Results:The expression level of miR-873 in both HCC tissues and cell lines was significantly down-regulared(P< 0.05),and miR-873 expression level was associated with tumor number,vein invasion,Edmondson-Steiner grading and tumor-node-metastasis (TNM) stage.Kaplan-Meier survival curves revealed that patients with lower miR-873 expression had a significantly re-duced survival rate than those with high miR-873 expression (P< 0.05),and miR-873 could be an independent prognostic factor for HCC patients.Results of Transwell assay suggested that miR-873 could supress invasion and metastasis of HCC cells.Conclusion:miR-873 was down-regulated in HCC,and was associated with tumor num-ber,vein invasion,Edmondson-Steiner grading and TNM stage and prognosis of HCC patients.miR-873 could su-press invasion and metastasis of HCC cells.