异甘草酸镁对奥沙利铂引起荷瘤裸鼠肝脏损伤的干预研究

摘要

目的：研究异甘草酸镁对奥沙利铂导致荷瘤裸鼠肝损伤模型的保护作用。方法：建立结肠癌HCT116细胞裸鼠皮下移植瘤模型，随机分成2组，每组8只，对照组为奥沙利铂组，实验组为奥沙利铂＋异甘草酸镁组。6天后处死裸鼠，腹主动脉取血测谷草转氨酶（AST）及谷丙转氨酶（ALT）的表达；取肝脏组织制作肝脏病理切片观察肝脏损伤情况，并制备肝脏组织匀浆，用比色法测量超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）及髓过氧化物酶（MPO）的表达情况。结果：成功建立了结肠癌HCT116细胞裸鼠皮下移植瘤模型；实验组肝窦血管损伤的发生率降低；血液中AST及ALT的表达实验组较对照组明显降低（P＜0．01）；肝脏SOD、GSH的表达实验组较对照组明显升高（P＜0．05）；MPO的表达实验组较对照组明显降低（P＜0．05）。结论：异甘草酸镁可以在一定程度上降低奥沙利铂引起的肝窦血管损伤发生率。%Objective:To evaluate the protective effect of isoglycyrrhizinate magnesium on liver injury model of tumor-bearing nude mice caused by oxaliplatin.Methods:Nude mice subcutaneous xenograft model was constructed by transplanting human colon carcinoma HCT1 16 cells,then divided into two groups of eight randomly,the control group for oxaliplatin group and the experimental group for oxaliplatin+isoglycyrrhizinate magnesium group.6 days lat-er,all nude mice were sacrificed.Collect abdominal aortic blood and test aspartate aminotransferase(AST)and ala-nine aminotransferase(ALT).The hepatic injury was observed by pathological section.The liver homogenate was pre-pared to detect the activity of superoxide dismutase(SOD),glutathione(GSH)and myeloperoxidase(MPO)expres-sion.Results:Nude mice subcutaneous xenograft model was constructed.The incidence of sinusoid vascular injury was lower in experimental group than the control group.The expression of AST and ALT were lower than control group(P<0.01).The expression of SOD and GSH were higher in experimental group than the control group,and MPO was lower(P<0.05).Conclusion:Isoglycyrrhizinate magnesium can reduced the incidence rate of hepatic injury caused by oxaliplatin.