胃癌组织中miR-134、VEGF表达与术后辅助化疗预后的关系

摘要

Objective To investigate the relationships between the expressions of miR-134 and vascular endothelial growth factor (VEGF) in gastric cancer with the prognosis of adjuvant chemotherapy gastric cancer after operation.Methods From May 2012 to July 2015,the tumor tissues and non-tumor tissues adjacent to cancer samples of 65 patients with gastric cancer who underwent radical surgery in the oncology department of our hospital were enrolled in this study.Quantitative polymerase chain reaction (qPCR) was used to detect the relative expression of miR-134 and VEGF in tissues,the expression of VEGF was detected by immunohistochemical staining,analyzing the relationships between the expression of miR-134 and VEGF with the clinicopathological characteristics of gastric cancer and the recurrence and metastasis of gastric cancer after adjuvant chemotherapy.Results The relative expression of miR-134 in gastric cancer tissues was lower than that in non-cancer tissues adjacent to cancer,and the relative expression and positive expression rate of VEGF was higher than that in non-cancer tissues adjacent to cancer;there was a negative correlation between the relative expression of miR-134 and VEGF mRNA (r =-0.765,P =0.003);their expression levels were correlated with the invasion of serosa,tumor node metastasis (TNM)stage,and lymph node metastasis of gastric cancer;during the follow-up period the recurrence and metastasis rate of 65 patients with gastric cancer was 41.54％ (27/65);the relative expression of miR-134 in tumor tissues of patients in recurrence and metastasis group was lower than that in non-recurrence and metastasis group (P ＜ 0.05),while the VEGF positive expression rate was higher than that in non-recurrence and metastasis group (P ＜ 0.05);the median tumor-free survival (DFS) in miR-134 low expression group was lower than that in miR-134 high expression group (P ＜0.05),and the DFS of patients with positive expression of VEGF was lower than that of patients with negative expression (P ＜ 0.05);infiltration of serosa,lymph node metastasis,low expression of miR-134,and positive expression of VEGF were risk factors for recurrence and metastasis of gastric cancer after adjuvant chemotherapy.Conclusions The low expression of miR-134 and high expression of VEGF in gastric cancer are closely related to the recurrence and metastasis of gastric cancer after adjuvant chemotherapy and DFS.%目的 探讨胃癌组织中miR-134、血管内皮生长因子(VEGF)表达与术后辅助化疗预后的关系.方法 收集2012年5月至2015年7月在内江市第二人民医院肿瘤科行根治性手术治疗的65例胃癌患者的肿瘤组织和癌旁非肿瘤组织样本,采用qPCR检测组织中miR-134、VEGF mRNA相对表达量,免疫组织化学染色法检测组织中VEGF蛋白表达,分析胃癌组织中miR-134、VEGF表达与胃癌临床病理特征及辅助化疗后复发转移的关系.结果 胃癌组织中miR-134相对表达量低于癌旁非肿瘤组织,VEGF mRNA相对表达量及阳性表达率高于癌旁非肿瘤组织;胃癌组织中miR-134相对表达量与VEGF mRNA相对表达量呈负相关(r=-0.765,P=0.003),两者表达水平与胃癌浸润浆膜层、TNM分期和淋巴结转移有关;65例胃癌患者随访期间复发转移率41.54％(27/65),复发转移组患者肿瘤组织中miR-134相对表达量低于未复发转移组患者(P＜0.05),VEGF阳性表达率高于未复发转移组患者(P＜0.05);miR-134低表达组中位无瘤生存期(DFS)低于miR-134高表达组(P＜0.05),VEGF阳性表达患者DFS低于阴性表达患者(P＜0.05);浸润浆膜层、淋巴结转移及miR-134低表达、VEGF阳性表达是胃癌辅助化疗后复发转移的危险因素.结论 胃癌组织中mi-134呈低表达,VEGF呈高表达,与胃癌辅助化疗后复发转移和DFS密切相关.