精喹·草除灵对泥鳅酯酶同工酶酶谱的影响

摘要

The effect of Quizalofop-P-ethyl · Benazolin on the expression of esterase (EST ) isozymes in five tissues including heart ,hepatopancreas ,spleen ,gill ,and muscle of orential weatherfish Misgurnus anguillicaudatus with body weight of 25 .20~28 .06 g were studied at water temperature of 23~25 ℃ by vertical polyacrylamide gel electrophoresis . The results showed that various numbers of EST strips showing different strength were observed in the different tissues of the orential weatherfish exposed to different concentrations of Quizalofop-P-ethyl · Benazolin for 24-96 h .The order of activity in tissues was arranged as :spleen > gill> heart > muscle > hepatopancreas .The EST expression was found to be increased in 24 h ,showing high-level of excitability ,and then decreased in 48 h or 72 h ,indicating that the expression was inhibited ,and increased again in 96 h ,which showed its adaptability .%用垂直平板聚丙烯酰胺凝胶电泳法，研究了在水温23～25℃下，精喹·草除灵对体质量25．20～28．06 g的泥鳅心脏、肝胰脏、脾脏、鳃和肌肉5种组织酯酶同工酶表达的影响。结果表明，经不同剂量（2．45、4．45、6．45 mg／L ）精喹·草除灵处理24～96 h后，泥鳅不同组织酯酶同工酶的酶带数量不同，表达的强度各异。酯酶的表达顺序为：脾脏＞鳃＞心脏＞肌肉＞肝胰脏，24 h酶活性比对照组显著增强，表现出应激性，48 h或72 h时表达减弱，酶表达受到抑制，96 h时又有所增强，表现出一定的适应性。