目的：观察细胞粘附分子E-cadherin在修复性牙本质形成中的免疫反应和免疫定位。方法：在大鼠上、下颌第一磨牙近中面制备单面洞，分别观察3、15和30d。标本行常规组织学处理。利用鼠抗E-cadherin和免疫组化SABC法，对标本进行标记，HE复染。结果：E-cadherin在术后3d成牙本质细胞染色较弱，牙髓细胞为阳性；15d后，成牙本质细胞样细胞和牙髓细胞染色阳性；30d后，成牙本质细胞样细胞出现极化，胞浆阳性染色明显。结论：E-cadherin可能在牙髓修复过程中参与了牙髓细胞的分化和极化过程。%AIM: To investigate the immunological reactivity of E- cadherin in the reparative dentinogenesis.METHODS: The cavity was prepared on the mesial surface of the first molar of Wistar rats. After the periods of 3, 15, 30days of post - operation, animal were sacrificed and paraffin sections were prepared through histological process. SABC method was used to detect the reactivity using rat anti - E- cadherin antibody. RESULTS: The reactivity was not evident in odontoblasts of 3 days group, but was evident in dental pulp cells; the positive staining was observed in odontoblast - like cells and pulp cells after 15 days; in 30 days, the polarized odontoblast- like cells were positively stained. CONCLUSION:E- cadherin may involve in the differentiation, and polarization of dental pulp cells.
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