SRC pY416在肝细胞癌中的表达与临床相关参数及肝内转移的关系

摘要

Background and purpose:Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. Intrahepatic recurrence is the main factor affecting its medium-term survival rate. Therefore, the search for the markers of metastasis is essential. This study aimed to evaluate the relationship of expression of tyrosine kinase phosphorylation Tyr416 of sarcoma (SRC pY416) in HCC with clinical parameters and prognosis. Methods:Immunohistochemical method and Western blot were used to detect the expression of non-receptor tyrosine kinase (SRC pY416) in 112 cases of HCC tissues and 40 cases of corresponding cancer adjacent normal liver tissues. Hepatitis B virus (HBV) DNA and alpha fetoprotein (AFP) in patients were detected with chemiluminescence. In the 12 months Follow-up of the study,the association between SRC pY416 expression and clinical parameters was analyzed. Results:SRC pY416 expressions in HCC (65.40±15.69) were higher than those in cancer adjacent normal liver tissues (11.25±2.73,P<0.001). The expressions of SRC pY416 were all associated with the age, the liver cirrhosis, the complete capsule, the tumor differentiation, the HBV DNA and the AFP value of the patients (P<0.01). 12 months after operation, single factor analysis showed that the recurrence was associated with the tumor differentiation, the HBV DNA, the AFP value and the expression of SRC pY416 of the patient (P<0.01). Multivariate analysis showed that the expression of SRC pY416 was an independent prognostic factor for recurrence and metastasis in patients with HCC in 12 months. Conclusion:SRC pY416 may play an important role in the metastasis of HCC. The expression of SRC pY416 may be the marker for HCC liver metastasis.%背景与目的:肝细胞癌(hepatocellular carcinoma,HCC)是最常见的恶性肿瘤之一,肝内复发转移是影响其中期生存率的主要因素,因此,转移标志物的寻找至关重要.该研究探讨HCC中肉瘤酪氨酸激酶磷酸化Tyr416(tyrosine kinase phosphorylation Tyr416 of sarcoma,SRC pY416)的表达与临床相关参数及肝内复发转移的关系.方法:采用免疫组织化学法和蛋白[质]印迹法(Western blot)检测112例HCC组织、40例相应癌旁正常肝脏组织中SRC pY416的表达,化学发光法检测患者乙型肝炎病毒DNA(hepatitis B virus-DNA,HBV-NDA)和甲胎蛋白(alpha fetoprotein,AFP),随访12个月,分析SRC pY416与HCC患者临床相关参数及预后的关系.结果:SRC pY416在HCC组织中的表达(65.40±15.69)均高于癌旁正常肝脏组织(11.25±2.73,P<0.001).SRC pY416的表达与患者年龄、肝硬化、包膜完整、分化程度、HBV-NDA及AFP均存在相关性(P<0.01).单因素分析显示,HCC患者术后12个月肝内复发转移与患者年龄、AFP表达、分化程度、是否携带HBV及SRC pY416蛋白表达有关(P<0.01),多因素分析显示,SRC pY416蛋白表达是HCC患者12个月肝内复发转移的独立危险因素.结论:SRC pY416可能在HCC转移中起重要作用,有望成为HCC肝内转移的分子标志物.