目的：明确苦参碱对 HaCaT 细胞 Bcl-2/ Bax 和 Fas/ FasL 表达的影响。方法：体外培养HaCaT 细胞，选择第二代细胞对数生长期 HaCaT 细胞作为研究对象，将细胞随机分为4组：苦参碱2 mg/ mL、10 mg/ mL 和50 mg/ mL 3组及对照组(加入相同体积的0.9％盐水)，孵育48 h 后，MTT 法测定各浓度下细胞增殖，RT-PCR 检测 Bcl-2/ Bax 和 Fas/ FasL 的表达。结果：与对照组相比，当苦参碱浓度为2 mg/ mL 时，HaCaT 细胞增殖活性无明显变化；Bcl -2、Bax、Fas、FasL 表达也无明显变化( P>0.05)。当苦参碱浓度为10 mg/ mL 时 HaCaT 细胞增殖活性较对照组明显下降(P<0.001)；Bcl-2表达明显下降，Bax 表达上升(P<0.001)；Fas、FasL 表达上升(P<0.05)。当苦参碱浓度为50 mg/ mL 时，MTT法检测 HaCaT 细胞增殖明显抑制(P<0.001)；同时 Bcl-2、 Bax、Fas 和 FasL 的变化与10 mg/ mL 时相似(P>0.05)。结论：苦参碱能够调控上皮细胞致炎因子的表达，抑制细胞的增殖。%To determine the effect of matrine on Bcl-2/ Bax and Fas/ FasL in keratinocytes in vitro. Methods: Second generation cultured HaCaT cells (logarithmic phase cells) were selected and divided into 4 groups:3 matrine groups (2 mg/ mL, 10 mg/ mL and 50 mg/ mL were used in each group) and the control group (0.9% Natrii Chloride). After 48-hour culture, the proliferation of HaCaT were detected by MTT and the levels of Bcl-2/ Bax and Fas/ FasL were measured by RT-PCR. Results: The viability of HaCaT cells was similar in 2 mg/ mL matrine group and control group (P>0.05). In 10 mg/ mL matrine group the proliferation of the cells was significantly decreased (P<0.001) and the Bcl-2 expression was remarkably reduced (P<0.001), while the expression of Bax, Fas and FasL was significantly increased (P<0.01 and P<0.05, respectively). When the concentration of matrine was increased to 50 mL, the viability and the expression of Bcl-2, Bax, Fas and FasL was similar to the results when 10 mL matrine was used. Conclusion: Matrine can inhibit HaCaT cells proliferation (at 10 mg/ mL or more) and may adjust expression of Bcl-2/ Bax and Fas/FasL in HaCaT cells.
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