青岛市胸科医院肺外结核的分枝杆菌培养及药敏试验结果分析

摘要

Objective To analyze the results of mycobacterial culture,bacterial type identification and drug susceptibility for mycobacterium tuberculosis in patients with extrapulmonary tuberculosis.Methods From January 2010 to December 2015,680 mycobacterium strains cultured from clinical specimens in Qingdao Chest Hospital,were reviewed.Results in 680 culture my-cobacteria were isolated and cultured,strain identification,anti -TB drug susceptibility testing using the absolute concentration method.Results Of 680 mycobacterium Clinical isolates,671 strains were identified as Mycobacterium tuberculosis(98.67%),9 strains were identified as non -tuberculous mycobacteria(1.32%).108 strains of drug -resistant Mycobacterium tubercu-losis were detected from 671 cases,in which 55 cases were a single drug resistance(8.19%)and 53 cases were MDR(7.89%).The sequence of Drug -resistant Mycobacterium tuberculosis pick descended isoniazid,streptomycin,rifampicin,ethambutol.Conclusion Mycobacterium tuberculosis is the main bacteria in culture positive specimens of extrapulmonary tuberculosis,and it can provide basis for drug resistance and drug treatment through studyingthe results of mycobacterial culture and drug susceptibility for extrapulmonary tuberculosis.%目的：分析肺外结核病患者的分枝杆菌培养、菌型鉴定及药敏试验结果。方法对青岛市胸科医院2010年1月———2015年12月培养所得的680株分枝杆菌进行分离培养，菌种鉴定，采用绝对浓度法进行抗结核药物的药敏实验。结果680株分枝杆菌中结核分枝杆菌671例（占98．67％），非结核分枝杆菌（NTM）9例（占1．32％）。671例结核分枝杆菌共检出耐药菌108例（占16．09％），其中单耐药55例（占8．19％）耐多药53株（占7．89％）。结核分枝杆菌的耐药顺位由高到低依次是异烟肼、链霉素、利福平、乙胺丁醇。结论肺外结核病标本培养阳性致病菌主要为结核分枝杆菌，对分枝杆菌培养及药敏结果进行分析，为肺外结核的耐药情况研究及药物治疗提供了依据。