Chemical Synthesis of Full-Length Parathyroid Hormone-Related Protein-(l-141)

摘要

Parathyroid hormone-related protein (PTHrP) is a 141-residue long protein (Figure 1) that plays a critical role in bone and tissue development. The intact protein can be obtained by recombinant methods, but shorter-length synthetic fragments, such as PTHrP(l-36), are used in most studies. Knowledge on the structural and functional properties of intact PTHrP is thus limited. No attempt at chemical synthesis of full-length PTHrP has so far been reported. We thus sought to produce full-length PTHrP(l-141) by chemical synthesis methods.