The voltage-gated proton channel (VSOP/Hv) contains four transmembrane domains that are analogous to the voltage-sensor in voltage-gated cation (Na~+, K~+, or Ca~(2+)) channels. Although VSOP contains no pore region (ion pathway), it allows protons to permeate the cell membrane from the inside by the sensing membrane potential [1,2]. VSOP(78-222) has sufficient functions such that it is comparable with the full-length VSOP [3]. To elucidate structural changes that are involved in voltage-sensing, we carried out the synthesis of VSOP(78-222).
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