collagen

摘要： The combination of therapies may be the promising future for the treatment of esthetic alterations more efficiently and in less time, in order to obtain an excellent result. Our objective was to evaluate the effectiveness of hydrolysed collagen associated with radiofrequency and electrostimulation in the treatment of flaccidity in the abdominal and gluteal regions. The sample consisted of 6 women, evaluated through evaluation protocols, questionnaires, dynamometry, ultrasound exams, and histological analysis. The volunteers were distributed into three subgroups: hydrolysed collagen group (G-1), radiofrequency + hydrolysed collagen group (G-2) and radiofrequency + hydrolysed collagen + electrostimulation group (G-3). The ultrasound analysis of the abdominal muscles showed an increase in muscle thickness in all groups, but not significant (p > 0.05). The histological analysis showed an evident increase in type I collagen in groups G-2 and G-3, with proliferating cells (Ki67+). The results of dynamometry showed significant values in different times (p < 0.05). There was a significant result in the infraumbilical perimetry analysis, when compared between the groups with 30 days (p = 0.03) and with 60 days (p = 0.04). All participants rated the treatment positively. The use of combined therapies has shown superior results for the treatment of abdominal and gluteal flaccidity when compared with its isolated use, being the combination of therapies important in the treatment of flaccidity.

摘要： In the glomeruli,mesangial cells produce mesangial matrix while podocytes wrap glomerular capillaries with cellular extensions named foot processes and tether the glomerular basement membrane(GBM).The turnover of the mature GBM and the ability of adult podocytes to repair injured GBM are unclear.The actin cytoskeleton is a major cytoplasmic component of podocyte foot processes and links the cell to the GBM.Predominant components of the normal glomerular extracellular matrix(ECM)include glycosaminoglycans,proteoglycans,laminins,fibronectin-1,and several types of collagen.In patients with diabetes,multiorgan composition of extracellular tissues is anomalous,including the kidney,so that the constitution and arrangement of glomerular ECM is profoundly altered.In patients with diabetic kidney disease(DKD),the global quantity of glomerular ECM is increased.The level of sulfated proteoglycans is reduced while hyaluronic acid is augmented,compared to control subjects.The concentration of mesangial fibronectin-1 varies depending on the stage of DKD.Mesangial type III collagen is abundant in patients with DKD,unlike normal kidneys.The amount of type V and type VI collagens is higher in DKD and increases with the progression of the disease.The GBM contains lower amount of type IV collagen in DKD compared to normal tissue.Further,genetic variants in theα3 chain of type IV collagen may modulate susceptibility to DKD and end-stage kidney disease.Human cellular models of glomerular cells,analyses of human glomerular proteome,and improved microscopy procedures have been developed to investigate the molecular composition and organization of the human glomerular ECM.

摘要： Objective: Comparison between semi-ablative and non-ablative radiofrequency for collagen stimulation. Method: Three volunteers with scheduled surgical procedures of abdominoplasty were selected. After the evaluation, the abdominal area was divided into three quadrants, with the right region receiving monopolar non-ablative radiofrequency (RF) application. In contrast, the left region received fractionated radiofrequency (FRF) application, using three different needle sizes (0.5, 1.5, and 3.0 mm), and the intermediate area was kept as a control. After 24 hours, surgery was performed, and the skin flaps were collected for histological and immunohistochemical analyses. Results: An increase in total collagen and type I collagen was observed in the treated groups. There was also an increase in the expression of COX-2, CD68 positive cells (macrophages), and lymphocyte markers (CD3, CD20, and NK-CD57). Furthermore, it was seen that only the use of 3.0-mm needles kept the channels open in the superficial tissue after 24 hours. There were no significant differences between the modalities. Conclusion: Non-ablative and sub-ablative radiofrequency have positive and effective results for flaccidity, demonstrating the success in collagen production. Furthermore, this study is the first to present channel opening and permanence time, which are important to optimize the action of drug delivery.

摘要： Based on the remarkable demand for facial reconstitute or reshape fillers due to the dermal defects arising from specific diseases,trauma,or aging,several natural or synthetic materials have been investigated.Among the evaluated materials,decellularized dermis is one of the most biocompatible choices for the aim of skin tissue regenerative approaches.On the other hand,Carboxymethyl Cellulose(CMC),a synthetic polysaccharide,with the desirable degradability,biomechanical stability,and nontoxicity seems to be an acceptable reinforcement agent for decellularized dermis.Thus,in this research,an injectable soft tissue filler contained of human-derived decellularized collagen and CMC was fabricated.The cell-removal approving was performed utilizing H&E staining assay.The biocompatibility of the prepared samples was confirmed by MTT assay.The rheology examination demonstrated the increased storage modulus and enhanced elastic property as a consequence of CMC presence.Furthermore,the required flow force of the collagen/CMC filler was decreased as a consequence of decreasing the viscosity and its injectability was improved.According to the provided biomechanical and biological results,it could be claimed that the collagen/CMC hydrogel is a suitable substitute filler for skin tissue engineering.

摘要： Gravity plays a central role in vertebrate development and evolution.Mechanotransduction involves the tensile tethering of veins and arteries,connections between the epidermis and dermis in skin,tensile stress concentrations that occur at tissue interfaces,cell-cell interactions,cell-collagen fiber stress transfer in extracellular matrix and fluid shear flow.While attention in the past has been directed at understanding the myriad of biochemical players associated with mechanotransduction pathways,less attention has been focused on determining the tensile mechanical behavior of tissues in vivo.Fibroblasts sit on the surface of collagen fibers in living skin and exert a retractile force on the fibers.This retractile force pulls against the tension in collagen fibers in skin.After fibroblast-collagen fiber interactions are altered either by changes in fibroblast adhesion or after formation of cancer associated fibroblasts,and changes in cell junctions,alterations in the retractive force leads to changes in mechanotransduction.The purpose of this paper is to present a model of tensile forces that occur at the fibroblast-collagen fiber interface and how these forces are important in extracellular matrix physiology in health and disease.

摘要： Objective:To explore the effects of a nano-formulation of curcumin(phytosomal curcumin)on the clinical and pathological symptoms of collagen-induced arthritis(CIA)in rats.Methods:Forty male Wistar rats were immunized with an emulsion containing bovine typeⅡcollagen and incomplete Freund's adjuvant and then administered phytosomal curcumin post-immunization.Clinical symptoms and histological analysis of the synovial tissues were performed.The effect of phytosomal curcumin on Th17 and Treg parameters was also evaluated.Results:Phytosomal curcumin reduced the clinical severity and paw swelling in CIA-induced rats,which was accompanied by a reduction in the number of inflammatory cell infiltration in the synovial tissue.Additionally,treatment with phytosomal curcumin significantly inhibited CIA-associated mediators as well as increased the anti-inflammatory mediators in comparison to the control groups.Conclusions:Phytosomal curcumin could improve CIA autoimmune responses and can be considered a potential candidate for the treatment of rheumatoid arthritis.

摘要： Collagen powder hydrolysates were reacted with a solution of commercial mimosa bark tannin extract.The mixture was prepared at ambient temperature and prepared at 80°C to determine what reactions,if any,did occur between the collagen protein through its amino acids and the polyphenolic condensed tannin.The reaction products obtained were analyzed by matrix assisted laser desorption ionization time-of-flight(MALDI ToF)mass spectrometry.Reactions between the two materials did appear to occur,with the formation of a relatively small proportion of covalent and ionic linkages at ambient temperature but a considerable proportion of covalent linkages tannin-protein amino acids and the disappearance of ionic bonds.The linkages between the two materials appeared to be by amination of the phenolic–OHs of the tannin by the amino groups of the non-skeletal side chains of arginine,and by esterification by the–COOH groups of glutamic and aspartic acid of the aliphatic alcohol-OH on the C3 site of the flavonoid units heterocycle of the tannin.The proportion of covalent linkages increases markedly and predominate with increasing temperatures.This tightening of the tannin-protein covalent network formed may be an additional contributing factor both to leather wear resistance and performance as well to leather shrinking when this is subjected to excessive temperatures.

摘要： BACKGROUND Coronary artery perforation is a rare but potentially life-threatening complication of percutaneous coronary intervention(PCI),however if recognized and managed promptly,its adverse consequences can be minimized.Risk factors include the use of advanced PCI technique(such as atherectomy and chronic total occlusion interventions)and treatment of severely calcified lesions.Large vessel perforation is usually treated with implantation of a covered stent,whereas distal and collateral vessel perforations are usually treated with embolization of coils,fat,thrombin,or collagen.We describe a novel and cost-effective method of embolisation using a cut remnant of a used angioplasty balloon that was successful in sealing a distal wire perforation.we advocate this method as a simple method of managing distal vessel perforation.CASE SUMMARY A 73-year-old male with previous coronary Bypass graft operation and recurrent angina on minimal exertion had undergone rotablation and PCI to his dominant left circumflex.At the end of the procedure there was evidence of wire perforation at the distal branch and despite prolonged balloon tamponade there continued to be extravasation and the decision was made to seal this perforation.A cut piece of an angioplasty balloon was used and delivered on the original angioplasty wire to before the perforation area and released which resulted in sealing of the perforation with no unwanted clinical consequences.CONCLUSION The use of a balloon remnant for embolization in coronary perforation presents a simple,efficient and cost-effective method for managing coronary perforations and may be an alternative for achieving hemostasis and preventing poor outcome.Prevention remains the most important part with meticulous attention to the distal wire position,particularly with hydrophilic wires.

摘要： Rheumatoid arthritis(RA)is an autoimmune disorder characterized by persistent inflammatory responses in target tissues and organs,resulting in the destruction of joints.Collagen type II(CII)-induced arthritis(CIA)is the most used animal model for human RA.Although BTN2A2 protein has been previously shown to inhibit T cell functions in vitro,its effect on autoimmune arthritis has not been reported.In this study,we investigate the ability of a recombinant BTN2A2-IgG2a Fc(BTN2A2-Ig)fusion protein to treat CIA.We show here that administration of BTN2A2-Ig attenuates established CIA,as compared with control Ig protein treatment.

摘要： BACKGROUND The extracellular matrix is the main component of the tumor microenvironment.Extracellular matrix remodels with the oncogenesis and development of tumors.Previous studies usually focused on the changes of proteins in normal colorectal tissues and colorectal cancers.Little is known about the changes in the extracellular matrix in different stages of colorectal cancer and the effects of these changes on the development of this cancer.AIM To test the changes of type I collagen,type IV collagen,matrix metalloproteinase-2(MMP-2),matrix metalloproteinase-9(MMP-9),and tissue inhibitor of metalloproteinase-3(TIMP-3)in different stages of colorectal cancer and the effects of these changes on the proliferation of cancer cells.METHODS The extracellular matrix from various stages of colorectal cancer and normal colon tissue was obtained by using acellular technology.We used proteomics to detect the differential expression of proteins between normal colon tissues and colorectal cancer tissues,and then we used Western blot to observe their expression in each stage of colorectal cancer and in normal colon tissue.By coculturing the extracellular matrix and HT29 colon cancer cells in vivo and in vitro,we tested the cancer cell proliferation rate in vitro by methyl thiazolyl tetrazolium(MTT)assay and in vivo by measuring the tumor volume.RESULTS The expression of type I collagen and MMP-2 increased with increased tumor stage.The expression of MMP-9 was higher in colorectal cancer tissues and was highest in stage III cancer.The expression of type IV collagen and TIMP-3 decreased with increased tumor stage.The proliferation rate of cancer cells in the extracellular matrix of colorectal cancer was higher than that in the extracellular matrix of the normal colon.CONCLUSION These data suggest that the extracellular matrix structure and composition become disorganized during the development of tumors,which is more conducive for the growth of cancer cells.