METHOD, DEVICE AND APPLICATION FOR SECOND NEAR-INFRARED REGION FLUORESCENCE IMAGING USING NEAR-INFRARED FLUORESCENT PROTEIN DERIVATIVE OR ANALOG

摘要

A method, device and application for the second near-infrared region fluorescence imaging using a near-infrared fluorescent protein derivative or an analog. Also provided is an expression of near-infrared fluorescent protein iRFP713 in the second near-infrared region. The near-infrared fluorescent protein has an emission wavelength in a near-infrared region greater than the emission spectrum of traditional GFP-type fluorescent proteins (approximately 400-600 nm) and is thus more suitable for in vivo bioimaging. The application has significant advantages in terms of penetration depth and spatial resolution compared to bioimaging using GFP-type proteins and imaging applications using near infrared proteins at the first near-infrared region (approximately 700-900 nm), and greatly improves the penetration depth and signal-to-noise ratio of in vivo imaging.