Mutated Artificial Genetic Circuit, and Method for Detection and Quantification of Phenolic Compounds Based on Communities of the Circuit and Machine Learning Algorithms

摘要

The present invention relates to a method for detecting and quantifying phenolic compounds using a mutant redesign gene circuit, more specifically, the 52nd methionine (M) of wild-type dmpR is substituted with isoleucine (I) or cysteine (C), or additionally wild-type dmpR Mutant redesign using a modified dmpR protein in which glutamine (Q) at position 71 is substituted with alanine (A), glutamic acid at position 135 (E) with lysine (K), or lysine (K) at position 188 with arginine (R) By constructing the genetic circuit, there is an effect of accurately detecting and quantifying even a small amount of various phenolic compounds.