Mutated Artificial Genetic Circuit, and Method for Detection and Quantification of Phenolic Compounds Based on Communities of the Circuit and Machine Learning Algorithms

摘要

The present invention is a method for detecting and quantifying a phenolic compound using a mutant redesigned genetic circuit, more specifically, the 52nd methionine (M) of wild type dmpR is substituted with isoleucine (I) or cysteine (C), or additionally of wild type dmpR. Mutation redesign using modified dmpR protein with 71th glutamine (Q) replaced by alanine (A), 135th glutamic acid (E) replaced by lysine (K), or 188th lysine (K) replaced by arginine (R) By constructing a genetic circuit, it is possible to accurately detect and quantify even small amounts of various phenolic compounds.