Serum-free culture medium for in vitro maturation of bovine oocytes and a method for culture of oocytes

摘要

A serum-free culture medium for in vitro maturation of bovine oocytes includes a Hepes-free M199 basal medium, fatty acid-free BSA, human menopausal gonadotrophin (HMG), 17β-estradiol, epidermal growth factor (EGF), L-cysteine, bFGF, Glutamax (100×), folic acid, cholic acid and CXCL12. A method of culturing bovine oocytes using such serum-free culture medium is provided.