COMPOSITION FOR IN VITRO MATURATION MEDIA OF MAMMALS OOCYTE AND METHOD FOR CULTURING MAMMALS OOCYTE IN VITRO USING THE SAME

摘要

The present invention relates to a composition for in vitro maturation culture of mammalian immature oocytes and to a method for in vitro culture of mammalian immature oocytes using the same. More particularly, the present invention relates to a method for in vitro culture of oocytes, which comprises azacerine, hadacidin, mycophenolic acid The present invention also relates to a method for in vitro culture of an immature oocyte of mammalian oocyte and a method for in vitro culture of a mammalian immature oocyte using the same, which can improve the fertility and the cleavage rate by using adenosine. In vitro maturation culture medium containing the purine synthesis inhibitor such as azacerine, hadacidin, mycophenolic acid, and adenosine is applied to immature oocytes. , It is possible to improve oocyte developmental competence of the immature oocyte and improve the fertility and fertility of the oocyte. The technique according to the present invention can be applied to restoration of ovarian function in a patient suffering from ovarian function deterioration due to in vitro fertilization, polycystic syndrome or other diseases.