Manufacturing method null of transformation microbe and heat-resistant gurukoshidaze which include the rearrangement body plasmid and the said plasmid which include heat-resistant gurukoshidaze gene DNA and said

摘要

PURPOSE:To mass produce heat-resistant beta-glucosidase through the cultivation at a temperature near the room one and purification by introducing the DNA which originates from a microorganism in Thermus and participates heat- resistant beta-glucosidase into Escherichia coli. CONSTITUTION:When the gene DNA participating the heat-resistant beta- glucosidase is isolated from a microorganism in Thermus, e.g., ZK-001 (FERM P-9184), a DNA having 4 recognition sites by restriction enzyme, BamH I; 2 sites by the restriction enzyme, Sac I, and 1 site by Kpn I. The DNA is bonded to a vector plasmid and introduced into Escherichia coli, then the microorganism is cultured to mass produce heat-resistant beta-glucosidase.