Manufacturing method of the growth hormone of the Pisces by the collection/symbol living thing and the said collection/symbol living thing which include the rearrangement body plasmid and the said rearrangement body plasmid of the growth hormone gene and said gene of the Pisces and growth promotion modulo null of the Pisces by the said growth

摘要

PURPOSE:To produce a large quantity of growth hormone of fishes at a low cost, by forming a recombinant plasmid containing a prescribed vector linked with a gene coding an amino acid sequence of a growth hormone polypeptide originated from a specific fish. CONSTITUTION:A DNA coding growth hormone of tuna is sectioned from a plasmid containing said DNA and is integrated into a vector DNA and the obtained recombinant DNA is introduced into a microorganism. The resultant transformant is cultured to produce and accumulate the tuna growth hormone polypeptide, which is separated from the cultured product. The plasmid containing the DNA coding tuna growth hormone is preferably pTTS339. The preferable vector DNA is pKK223-3, pKK233-2, etc.