DO-stat fed-batch culture method for mass production of granulocyte colony stimulating factor

摘要

The present invention relates to a DO-stat fed-batch culture method for mass production of granulocyte colony stimulating factor (G-CSF). More specifically, the present invention relates to a method for producing recombinant Escherichia coli comprising an inducible G-CSF expression vector using an L-arabinose operon of Salmonella strains in a batch culture in an initial culture medium, wherein the amount of dissolved oxygen is rapidly increased Arabinose at the time of starting the oil-feed culture while culturing the oil-in-oil-in-oil-in-oil culture medium. According to the DO-stat fed-batch fermentation method of the present invention, glycerol is used as a carbon source in the ash fermentation section in which the carbon source is present at a high concentration to reduce the inhibitory effect of arabinose operon and accumulation of acetic acid, In addition, L-arabinose was added to the cells at the beginning of the cell culture to prevent the expression of G-CSF by maintaining the low glucose concentration below the minimum level of expression inhibition. The stability of plasmids and the expression level of G-CSF can be stably maintained at a high level, resulting in mass production of G-CSF at an inexpensive production cost.