PURPOSE: Provided is a method of preparing an egg by inoculating a mixed bacteria emulsion of E. Coli, Rotavirus and Astrovirus into a chicken and then growing it, thereby directly treating and preventing diarrhea and enteritis causing the diarrhea. CONSTITUTION: An egg is prepared by the steps of: preparing a mixed bacteria emulsion by emulsifying an antigen of E. Coli, Rotavirus and Astrovirus with aluminium hydroxide; inoculating 0.5ml mixed bacteria emulsion into a chicken one time; preparing a mixed bacteria emulsion by emulsifying the three antigens with an emulsion adjuvant(ISA25); inoculating the mixed bacteria emulsion into a chicken in an amount of 0.5ml two times at two week intervals; and then producing an egg containing anti-E. Coli immunoprotein(IgY), anti-Rotavirus immunoprotein(IgY) and anti-Astrovirus immunoprotein(IgY) from the chicken.
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