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Single-cell reporter assay for monitoring high spatiotemporal resolution gene expression patterns
Single-cell reporter assay for monitoring high spatiotemporal resolution gene expression patterns
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机译:用于监测高时空分辨率基因表达模式的单细胞报告基因检测
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摘要
The invention relates to a double-stranded polynucleotide comprising on its positive strand considered from its 5' end to its 3' end, (i) a promoter of a gene of interest or several promoters of various genes of interest selected among genes which are endogenous to a determined cell, and, (ii) one or several barcode(s) wherein each barcode contains at least one barcode unit formed of at least one, especially of multiple, recognition binding sites each binding site being composed of a nucleotide sequence, and wherein each of said barcode(s) is under the control of at least one of said promoter(s) for transcription. It further concerns use of said polynucleotide to monitor gene expression patterns in living cells, especially in single cells, with a rapid and high spatio-temporal resolution.
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